Listeria sp. p60 Antibody [p6017]

Product Specifications

Background

Listeria monocytogenes is a intracellular bacterium that is the causative agent of Listeriosis. It is one of the most virulent foodborne pathogens. Listeria species are known to secrete a 60-kDa protein collectively termed p60, which is encoded by the iap (invasion-associated-protein) gene and secreted in large quantites into the growth media. p60 is a highly immunogenic murein hydrolase that is essential for cell division.

NCBI Gene ID

985140

Swiss Prot

P21171

Accession Number

1171970

Host

Mouse

Reactivity

Listeria monocytogenes, Listeria sp.

Immunogen

Recombinant Listeria spp. p60.

Clonality

Monoclonal

Clone

P6017

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes L. monocytogenes p60 protein. Weakly cross-reacts with other Listeria species (L. innocua, L. ivanovii) .

Applications Notes

ELISA: (direct or indirect: 1:5,000-1:10,000) . Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.