Resistin Antibody [RRES 07]

Product Specifications

Background

The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELMalpha/FIZZ 1 and RELMbeta/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.

NCBI Gene ID

246250

Swiss Prot

Q8K4J7

Accession Number

81866779

Host

Mouse

Reactivity

Rat

Immunogen

Recombinant rat resistin.

Clonality

Monoclonal

Clone

RRES 07

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes rat resistin. Detects a band of ~10kDa by Western blot. Does not cross-react with human and mouse resistin.

Applications Notes

ELISA: (direct or indirect: 1:5,000-1:10,000) . Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.