Adiponectin Antibody [MADI 04]
Product Specifications
Background
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
NCBI Gene ID
11450
Swiss Prot
Q60994
Accession Number
408359957
Host
Rat
Reactivity
Mouse
Immunogen
Recombinant mouse adiponectin.
Clonality
Monoclonal
Clone
MADI 04
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Obesity
Concentration
1 mg/ml
Buffer
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 1 year after receipt when stored at -20˚ C.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=36-004
Fragment
IgG1, kappa
Specificity
Recognizes mouse adiponectin. Detects a band of ~30kDa by Western blot. Weakly cross-reacts with human and rat adiponectin.
Applications Notes
ELISA: (direct and indirect: 1:5,000-1:10,000) . Immunoprecipitation: (1-5ul per sample in a final reaction volume of 500-1,000ul) . Western blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.
Symbol
Adipoq
NCBI Official Name
Adiponectin, C1Q and collagen domain containing
NCBI Organism
Mus musculus
Other Product Names
ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa
Tested Applications
ELISA, IP, WB
Protein ID
Q60994
Physical Properties
Liquid
Curated Selection
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