Adiponectin Antibody [MADI 1147]

Product Specifications

Background

ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

NCBI Gene ID

11450

Swiss Prot

Q60994

Accession Number

408359957

Host

Mouse

Reactivity

Mouse

Immunogen

Recombinant mouse adiponectin.

Clonality

Monoclonal

Clone

MADI 1147

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes mouse adiponectin. Detects a band of ~30kDa by Western blot. Weakly cross-reacts with human and rat adiponectin.

Applications Notes

ELISA: (direct and indirect: 1:5,000-1:10,000) . Immunoprecipitation: (1-5ug per sample in a final reaction volume of 500-1000ul) . Western blot: (1:5,000-1:10,000 using ECL; suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.