Adiponectin Antibody [HADI 773]

Product Specifications

Background

ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

NCBI Gene ID

9370

Swiss Prot

Q15848

Accession Number

295317372

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human adiponectin.

Clonality

Monoclonal

Clone

HADI 773

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Concentration

1 mg/ml

Buffer

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at -20˚ C.

Fragment

IgG1, kappa

Specificity

Recognizes human adiponectin. Detects a band of ~30kDa by Western blot. Cross-reactivity with other species not tested.

Applications Notes

ELISA: (direct and indirect: 1:5,000-1:10,000) . Immunohistochemistry: (paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature) . Western blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.