NSUN2 Antibody

Product Specifications

Background

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) (Brzezicha et al., 2006 [PubMed 17071714]) .

NCBI Gene ID

54888

Swiss Prot

Q08J23

Accession Number

Q08J23

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This NSUN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-451 amino acids from the Central region of human NSUN2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

86 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

NSUN2

NCBI Official Name

TRNA (cytosine (34) -C (5) ) -methyltransferase

NCBI Organism

Homo sapiens

Background Reference 01

Bailey, S.D., et al. Diabetes Care (2010) In press :

Background Reference 02

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Background Reference 03

Frye, M., et al. Cancer Lett. 289 (1) :71-80 (2010)

Background Reference 04

Talmud, P.J., et al. Am. J. Hum. Genet. 85 (5) :628-642 (2009)

Other Product Names

TRNA (cytosine (34) -C (5) ) -methyltransferase, Myc-induced SUN domain-containing protein, Misu, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-) -methyltransferase, tRNA methyltransferase 4 homolog, hTrm4, NSUN2, SAKI, TRM4