SETDB1 Antibody

Product Specifications

Background

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su (var) 3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.

NCBI Gene ID

9869

Swiss Prot

Q15047

Accession Number

Q15047

Host

Rabbit

Reactivity

Human

Immunogen

This SETDB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1193-1225 amino acids from the C-terminal region of human SETDB1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Mouse, Zebrafish, Xenopus

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

143 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

SETDB1

NCBI Official Name

Histone-lysine N-methyltransferase SETDB1

NCBI Organism

Homo sapiens

Background Reference 01

Ichimura, T., et al., J. Biol. Chem. 280 (14) :13928-13935 (2005) .

Background Reference 02

Sarraf, S.A., et al., Mol. Cell 15 (4) :595-605 (2004) .

Background Reference 03

Wang, H., et al., Mol. Cell 12 (2) :475-487 (2003) .

Background Reference 04

Schultz, D.C., et al., Genes Dev. 16 (8) :919-932 (2002) .

Other Product Names

Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, ESET, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SETDB1, KIAA0067, KMT1E