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ATP6V0C Antibody

Product Specifications

Background

ATP6V0C is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. ATP6V0C encodes the V0 subunit c.

NCBI Gene ID

527

Swiss Prot

P27449

Accession Number

P27449

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This ATP6V0C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-126 amino acids from the C-terminal region of human ATP6V0C.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: C. Elegans, Bovine, Drosophila, Rat

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=55-198

Calculated Molecular Weight

16 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

ATP6V0C

NCBI Official Name

V-type proton ATPase 16 kDa proteolipid subunit

NCBI Organism

Homo sapiens

Background Reference 01

O'Callaghan, K.M., et al. J. Biol. Chem. 285 (1) :381-391 (2010)

Background Reference 02

You, H., et al. Cancer Lett. 280 (1) :110-119 (2009)

Background Reference 03

Lee, I., et al. J. Biol. Chem. 279 (51) :53007-53014 (2004)

Background Reference 04

Morel, N. Biol. Cell 95 (7) :453-457 (2003)

Other Product Names

V-type proton ATPase 16 kDa proteolipid subunit, V-ATPase 16 kDa proteolipid subunit, Vacuolar proton pump 16 kDa proteolipid subunit, ATP6V0C, ATP6C, ATP6L, ATPL

Tested Applications

WB

Protein ID

137479

Physical Properties

Liquid

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