NKX2.1 Antibody [SPM150]

Product Specifications

Background

Recognizes a protein of 40kDa, identified as NKX2.1 or Thyroid transcription factor-1 (TTF-1) . TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of NKX2.1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. Reactivity is also seen in thyroid malignancies.

NCBI Gene ID

7080

Swiss Prot

P43699

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Full length rat recombinant protein was used as the immunogen for this NKX2.1 antibody.

Clonality

Monoclonal

Clone

SPM150

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1, kappa

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.