Bax Antibody [SPM336]
Product Specifications
Background
Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
NCBI Gene ID
581
Swiss Prot
Q07812
Host
Mouse
Reactivity
Human, Monkey
Immunogen
Amino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.
Clonality
Monoclonal
Clone
SPM336
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Apoptosis, Autophagy, Cancer
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=34-157
Fragment
IgG1
Specificity
Does not react with mouse and rat
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
BAX
NCBI Official Name
BCL2-associated X protein
NCBI Organism
Homo sapiens
Other Product Names
Bax Antibody: BCL2L4, BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4
Tested Applications
Flow, IF, WB, IHC
Physical Properties
Liquid
Curated Selection
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