Glial Fibrillary Acidic Protein Antibody [SPM248]

Product Specifications

Background

Glial Fibrillary Acidic Protein is specifically found in astroglia, making it a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Ab to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

NCBI Gene ID

2670

Swiss Prot

P14136

Host

Mouse

Reactivity

Human, Mouse, Rat, Bovine, Pig, Rabbit, Chicken

Immunogen

GFAP isolated from pig spinal cord was used as the immunogen for this Glial Fibrillary Acidic Protein antibody.

Clonality

Monoclonal

Clone

SPM248

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.