NSE Antibody [NSEL1-1]

Product Specifications

Background

Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NCBI Gene ID

2026

Swiss Prot

P09104

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 416-433 of human Neuron-specific Enolase were used as the immunogen for this NSE antibody.

Clonality

Monoclonal

Clone

NSEL1-1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Obesity, Neuroscience, Stem Cell

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG2b, kappa

Applications Notes

1. Staining of FFPE tissue requires boiling sections in 10mM Citrate Buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Symbol

ENO2

NCBI Official Name

Enolase 2 (gamma, neuronal)

NCBI Organism

Homo sapiens

Other Product Names

ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neurone-specific enolase, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE