Recombinant WT1 Antibody [RMWT1-1]

Product Specifications

Background

Wilms tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

NCBI Gene ID

7490

Swiss Prot

P19544

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant protein was used as the immunogen for the WT1 antibody.

Clonality

Recombinant Monoclonal

Clone

RMWT1-1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cell Cycle, Cancer, Apoptosis

Purification

Protein A affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG, k

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.