Nucleolin Antibody [NPC23-2]
Product Specifications
Background
Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
NCBI Gene ID
4691
Swiss Prot
P19338
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Recombinant human protein was used as the immunogen for the Nucleolin antibody.
Clonality
Monoclonal
Clone
NPC23-2
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Fragment
IgG1
Applications Notes
1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
NCL
NCBI Official Name
Nucleolin
NCBI Organism
Homo sapiens
Other Product Names
Nucleolin, Protein C23, NCL
Tested Applications
Flow, IHC-P, WB, IF
Physical Properties
Liquid
Frequently Asked Questions
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