Nucleolin Antibody [NPC23-2]

Product Specifications

Background

Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

NCBI Gene ID

4691

Swiss Prot

P19338

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Clonality

Monoclonal

Clone

NPC23-2

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1

Applications Notes

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.