TGF alpha Antibody [TFGFa-1]

Product Specifications

Background

Transforming growth factor alpha is a protein that in humans is encoded by the TGFA gene. As a member of the epidermal growth factor (EGF) family, TGFa is a mitogenic polypeptide. The protein becomes activated when binding to receptors capable of protein kinase activity for cellular signaling. TGFa is a transforming growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. [Wiki]

NCBI Gene ID

7039

Swiss Prot

P01135

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length TGF protein was used as the immunogen for the TGF alpha antibody. (3)

Clonality

Monoclonal

Clone

TFGFa-1

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies, Antibody Pairs

Field of Research

Growth Factors

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1, kappa

Applications Notes

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. 3. The epitope is known to be in the C-termial region of the protein.