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PGP9.5 Antibody [UBCE-L1]

Product Specifications

Background

UCHL1/PGP9.5 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of PGP9.5 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is abundantly present in all neurons (accounts for 1-2% of total brain protein), expressed specifically in neurons and testis/ovary. [Wiki]

NCBI Gene ID

7345

Swiss Prot

P09936

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant human protein was used as the immunogen for the PGP9.5 antibody.

Clonality

Monoclonal

Clone

UBCE-L1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Neuroscience

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1, kappa

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Symbol

UCHL1

NCBI Official Name

Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)

NCBI Organism

Homo sapiens

Other Product Names

Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 6---, Neuron cytoplasmic protein 95, PGP 95, PGP95, Ubiquitin thioesterase L1, UCHL1

Tested Applications

WB, IHC-P

Physical Properties

Liquid

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