Smooth Muscle Actin Antibody [SMAT2-1]
Product Specifications
Background
Actins are a family of proteins which are highly conserved and play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. [RefSeq]
NCBI Gene ID
59
Swiss Prot
P62736
Host
Mouse
Reactivity
Human, Rat
Immunogen
Recombinant full-length human ACTA2 protein was used as the immunogen for the Smooth Muscle Actin antibody.
Clonality
Monoclonal
Clone
SMAT2-1
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Fragment
IgG2a, kappa
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
ACTA2
NCBI Official Name
Actin, alpha 2, smooth muscle, aorta
NCBI Organism
Homo sapiens
Other Product Names
ACTA2, ACTSA, actin, alpha 2, smooth muscle, aorta, alpha-cardiac actin, ACTVS, GIG46
Tested Applications
IF, IHC-P
Physical Properties
Liquid
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