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Bax Antibody [ARBX-1]

Product Specifications

Background

BCL2 associated X protein/Bax accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. [UniProt]

NCBI Gene ID

581

Swiss Prot

Q07812

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the Bax antibody.

Clonality

Monoclonal

Clone

ARBX-1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Autophagy, Cancer

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1

Specificity

Does not react with mouse and rat

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Symbol

BAX

NCBI Official Name

BCL2-associated X protein

NCBI Organism

Homo sapiens

Other Product Names

Bax Antibody: BCL2L4, BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

Tested Applications

WB, IF, IHC-P

Physical Properties

Liquid

Frequently Asked Questions

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