Neurofilament (light chain) Antibody [MSI27-1]
Product Specifications
Background
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. [RefSeq]
NCBI Gene ID
4747
Swiss Prot
P07196
Host
Mouse
Reactivity
Human, Rat
Immunogen
Recombinant human neurofilament light chain protein was used as the immunogen for the Neurofilament antibody.
Clonality
Monoclonal
Clone
MSI27-1
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Neuroscience, Signal Transduction, Stem Cell
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=33-960
Fragment
IgG1
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
NEFL
NCBI Official Name
Neurofilament, light polypeptide
NCBI Organism
Homo sapiens
Other Product Names
NEFL, 68 kDa neurofilament protein, CMT1F, CMT2E, NF68, NF-L, NFL, Neurofilament subunit NF-L
Tested Applications
IF, IHC-P
Physical Properties
Liquid
Curated Selection
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