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Neurofilament (light chain) Antibody [MSI27-1]

Product Specifications

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. [RefSeq]

NCBI Gene ID

4747

Swiss Prot

P07196

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant human neurofilament light chain protein was used as the immunogen for the Neurofilament antibody.

Clonality

Monoclonal

Clone

MSI27-1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Signal Transduction, Stem Cell

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Symbol

NEFL

NCBI Official Name

Neurofilament, light polypeptide

NCBI Organism

Homo sapiens

Other Product Names

NEFL, 68 kDa neurofilament protein, CMT1F, CMT2E, NF68, NF-L, NFL, Neurofilament subunit NF-L

Tested Applications

IF, IHC-P

Physical Properties

Liquid

Frequently Asked Questions

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