CD45RA Antibody [CDLA45RA-1]

Product Specifications

Background

CD45RA is an isoform of the human leukocyte common antigen (CD45) . Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells.

NCBI Gene ID

5788

Swiss Prot

P08575

Host

Mouse

Reactivity

Human

Immunogen

Stimulated human leukocytes were used as immunogen for this anti-CD45RA antibody.

Clonality

Monoclonal

Clone

CDLA45RA-1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Immunology, Neuroscience, Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG2a, kappa

Applications Notes

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.