FOXP3 Antibody [FHBP3-1]

Product Specifications

Background

The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 is essential for normal immune homeostasis. It represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

NCBI Gene ID

50943

Swiss Prot

Q9BZS1

Host

Mouse

Reactivity

Human, Mouse

Immunogen

An N-terminal recombinant protein fragment was used as the immunogen for this FOXP3 antibody.

Clonality

Monoclonal

Clone

FHBP3-1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1, kappa

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.