Melan-A Antibody [MRT3V]
Product Specifications
Background
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.
NCBI Gene ID
2315
Swiss Prot
Q16655
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Recombinant human protein was used as the immunogen for the Melan-A antibody.
Clonality
Monoclonal
Clone
MRT3V
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Immunology
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=33-873
Fragment
IgG1
Applications Notes
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
MLANA
NCBI Official Name
Melan-A
NCBI Organism
Homo sapiens
Other Product Names
MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1
Tested Applications
IF, IHC-P
Physical Properties
Liquid
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