Recombinant CD34 Antibody [HPCA1/1806R]

Product Specifications

Background

This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.

NCBI Gene ID

947

Swiss Prot

P28906

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.

Clonality

Recombinant Monoclonal

Clone

HPCA1/1806R

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction

Purification

Protein A affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG, k

Applications Notes

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.