Cytokeratin 5/8 Antibody [SPM268]
Product Specifications
Background
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa) . Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
NCBI Gene ID
38523856
Swiss Prot
P13647, P05787
Host
Mouse
Reactivity
Human, Pig, Mouse, Rat, Hamster, Bovine, Dog, Sheep, Ferret
Immunogen
A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.
Clonality
Monoclonal
Clone
SPM268
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Fragment
IgG1, kappa
Specificity
Does not react with Chicken & Xenopus.
Applications Notes
1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at RT
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
KRT5, KRT8
NCBI Official Name
Keratin 5, keratin 8
NCBI Organism
Homo sapiens
Tested Applications
Flow, IF, WB, IHC-P
Physical Properties
Liquid
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