GRP94 Antibody [HSP90B1/1192]

Product Specifications

Background

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96) . Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90) . This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

NCBI Gene ID

7184

Swiss Prot

P14625

Host

Rat

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the GRP94 antibody.

Clonality

Monoclonal

Clone

HSP90B1/1192

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Obesity, Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG2a, kappa

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Symbol

HSP90B1

NCBI Official Name

Heat shock protein 90kDa beta (Grp94), member 1

NCBI Organism

Homo sapiens

Other Product Names

Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, HSP90B1, GRP94, TRA1