TGFa Antibody [TGFA/1119]

Product Specifications

Background

This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

NCBI Gene ID

7039

Swiss Prot

P01135

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length TGF protein was used as the immunogen for the TGFa antibody.

Clonality

Monoclonal

Clone

TGFA/1119

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies, Antibody Pairs

Field of Research

Growth Factors

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1, kappa

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.