CD99 Antibody [HO36-1.1]

Product Specifications

Background

CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. [UniProt]

NCBI Gene ID

4267

Swiss Prot

P14209

Host

Mouse

Reactivity

Human, Rat

Immunogen

Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.

Clonality

Monoclonal

Clone

HO36-1.1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

PEG precipitation

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgM, kappa

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Symbol

CD99

NCBI Official Name

CD99 molecule

NCBI Organism

Homo sapiens

Other Product Names

CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 molecule, E2 antigen, HBA71