MFG-E8 Antibody [SPM291]
Product Specifications
Background
Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
NCBI Gene ID
4240
Swiss Prot
Q08431
Host
Mouse
Reactivity
Human
Immunogen
A human milk fat globule membrane preparation was used as the immunogen for the MFG-E8 antibody.
Clonality
Monoclonal
Clone
SPM291
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle, Cell Cycle, Obesity
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=33-437
Fragment
IgG1, kappa
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
MFGE8
NCBI Official Name
Milk fat globule-EGF factor 8 protein
NCBI Organism
Homo sapiens
Other Product Names
Lactadherin, Breast epithelial antigen BA46, HMFG, MFGM, Milk fat globule-EGF factor 8, MFG-E8, SED1, Lactadherin short form, Medin, MFGE8
Tested Applications
WB, Flow, IF, IHC-P
Physical Properties
Liquid
Curated Selection
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