GFAP Antibody [ASTRO/789]
Product Specifications
Background
This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
NCBI Gene ID
2670
Swiss Prot
P14136
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Pig, Rabbit, Chicken
Immunogen
Recombinant protein was used as the immunogen for the GFAP antibody.
Clonality
Monoclonal
Clone
ASTRO/789
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Neuroscience, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=33-367
Fragment
IgG1
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
GFAP
NCBI Official Name
Glial fibrillary acidic protein
NCBI Organism
Homo sapiens
Other Product Names
Glial fibrillary acidic protein, GFAP, GFAP
Tested Applications
Flow, IF, WB, IHC-P
Physical Properties
Liquid
Curated Selection
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