P27Kip1 Antibody [KIP1/769]
Product Specifications
Background
This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.
NCBI Gene ID
1027
Swiss Prot
P46527
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat
Immunogen
Recombinant human protein was used as the immunogen for the p27Kip1 antibody.
Clonality
Monoclonal
Clone
KIP1/769
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Cell Cycle
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Fragment
IgG1, kappa
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
CDKN1B
NCBI Official Name
Cyclin-dependent kinase inhibitor 1B (p27, Kip1)
NCBI Organism
Homo sapiens
Other Product Names
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1
Tested Applications
Flow, IF, WB, IHC-P
Physical Properties
Liquid
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