CD45RO Antibody [T200/797]
Product Specifications
Background
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) . This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
NCBI Gene ID
5788
Swiss Prot
P08575
Host
Mouse
Reactivity
Human
Immunogen
Human T lymphocytes were used as immunogen for this CD45RO antibody.
Clonality
Monoclonal
Clone
T200/797
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Immunology, Neuroscience, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=33-304
Fragment
IgG2a, kappa
Applications Notes
1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
PTPRC
NCBI Official Name
Protein tyrosine phosphatase, receptor type, C
NCBI Organism
Homo sapiens
Other Product Names
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
Tested Applications
WB, Flow, IF, IHC
Physical Properties
Liquid
Curated Selection
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