Acidic Cytokeratin Antibody [AE1]

Product Specifications

Background

This antibody is specific for the 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), and 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Many studies have shown the usefulness of CKs as markers in cancer research and tumor diagnosis, as epithelial tumors generally maintain the same CK expression patterns as their counterpart normal tissue. Clone AE1 is commonly used with clone AE3 as a pan cytokeratin antibody cocktail (Cat No V2330) . Cat No V2330

NCBI Gene ID

3858

Swiss Prot

P13645

Host

Mouse

Reactivity

Human, Monkey, Bovine, Dog, Rabbit, Mouse, Rat, Chicken, Turtle

Immunogen

Solubilized keratin extract from human stratum corneum

Clonality

Monoclonal

Clone

AE1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

Protein G purified

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.