BrdU Antibody [BRD469]
Product Specifications
Background
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
NCBI Gene ID
NotApplicable
Host
Mouse
Reactivity
All species
Immunogen
Bromodeoxyuridine conjugated to KLH was used as the immunogen.
Clonality
Monoclonal
Clone
BRD469
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Fragment
IgG1
Applications Notes
1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.
Tested Applications
IHC, Flow, IF
Physical Properties
Liquid
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