Biotin Antibody [Hyb-8]
Product Specifications
Background
This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.
Host
Mouse
Reactivity
All species
Immunogen
Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.
Clonality
Monoclonal
Clone
Hyb-8
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Fragment
IgG1
Applications Notes
1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Tested Applications
WB, IHC, Flow, IF, CM
Physical Properties
Liquid
Frequently Asked Questions
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