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Biotin Antibody [Hyb-8]

Product Specifications

Background

This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.

Host

Mouse

Reactivity

All species

Immunogen

Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.

Clonality

Monoclonal

Clone

Hyb-8

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1

Applications Notes

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Tested Applications

WB, IHC, Flow, IF, CM

Physical Properties

Liquid

Frequently Asked Questions

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