TGF alpha Antibody [1E8-G6]

Product Specifications

Background

TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.

NCBI Gene ID

7039

Swiss Prot

P01135

Host

Mouse

Reactivity

Human, Rabbit, Zebrafish

Immunogen

A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

1E8-G6

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies, Antibody Pairs

Field of Research

Growth Factors

Purification

PEG precipitation

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgM, kappa

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.