Gp100 Antibody [HMB45]
Product Specifications
NCBI Gene ID
6490
Swiss Prot
P40967
Host
Mouse
Reactivity
Human
Immunogen
The extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.
Clonality
Monoclonal
Clone
HMB45
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Obesity, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Fragment
IgG1, kappa
Specificity
Does not react with dog, rat
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
PMEL
NCBI Official Name
Premelanosome protein
NCBI Organism
Homo sapiens
Other Product Names
Melanocyte protein PMEL, ME20-M, ME20M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, M-alpha, 95 kDa melanocyte-specific secreted glycoprotein, P26, Secreted melanoma-associated ME20 antigen, ME20-S, ME20S, M-beta, PMEL, D12S53E, PMEL17, SILV
Tested Applications
WB, Flow, IHC, IF
Physical Properties
Liquid
Curated Selection
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