CD45RO Antibody [UCHL-1]
Product Specifications
Background
This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31) . The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
NCBI Gene ID
5788
Swiss Prot
P08575
Host
Mouse
Reactivity
Human, Chimpanzee, Marmoset
Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as the immunogen for the CD45RO antibody.
Clonality
Monoclonal
Clone
UCHL-1
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Immunology, Neuroscience, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=33-194
Fragment
IgG2a, kappa
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
PTPRC
NCBI Official Name
Protein tyrosine phosphatase, receptor type, C
NCBI Organism
Homo sapiens
Other Product Names
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
Tested Applications
WB, Flow, IHC, IF
Physical Properties
Liquid
Curated Selection
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