Neurofilament Antibody [NF421]

Product Specifications

Background

Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H) . The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.

NCBI Gene ID

4744

Swiss Prot

P12036

Host

Mouse

Reactivity

Human, Mouse, Rat, Pig, Chicken

Immunogen

Human recombinant Neurofilament protein was used as the immunogen. (1)

Clonality

Monoclonal

Clone

NF421

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Signal Transduction, Stem Cell

Purification

Protein G purified Neurofilament antibody

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1

Applications Notes

1. The NF421 antibody is specific to the heavy subunit. 2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.