Kappa Light Chain Antibody [KLC709]
Product Specifications
Background
This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
NCBI Gene ID
3514
Swiss Prot
P01834, P01601
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human Kappa light chain was used as the immunogen for this anti-Kappa light chain antibody.
Clonality
Monoclonal
Clone
KLC709
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Immunology
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=33-151
Fragment
IgG1, kappa
Applications Notes
1. Staining of FFPE tissues requires boiling sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
IGKC
NCBI Official Name
Immunoglobulin kappa constant
NCBI Organism
Homo sapiens
Tested Applications
WB, IHC, Flow
Physical Properties
Liquid
Curated Selection
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