CD50 Antibody [186-2G9]
Product Specifications
Background
This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI) . It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.
NCBI Gene ID
3385
Swiss Prot
P32942
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for this CD50 antibody.
Clonality
Monoclonal
Clone
186-2G9
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Immunology, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Fragment
IgG2b, kappa
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
ICAM3
NCBI Official Name
Intercellular adhesion molecule 3
NCBI Organism
Homo sapiens
Other Product Names
Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, CD50, ICAM3
Tested Applications
Flow, IHC, IF, Blocking
Physical Properties
Liquid
Curated Selection
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