HSP60 Antibody [LK1]
Product Specifications
Background
This antibody recognizes a 60kDa protein, identified as the heat shock protein 60. The antibody's epitope is localized between amino acids 383-447 of human HSP60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp’s), more appropriately called as stress response proteins (srp’s) . HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog. Clone LK1 antibody, unlike LK2 antibody, recognizes only the mammalian (not bacterial) HSP60 and is useful in distinguishing HSP60 from mammals and bacteria.
NCBI Gene ID
3329
Swiss Prot
P10809
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster, Sheep, Rabbit, Bovine, Dog, Pig, Monkey, Chicken, Frog, Drosophila
Immunogen
Recombinant human HSP60 was used as the immunogen for this antibody.
Clonality
Monoclonal
Clone
LK1
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=33-141
Fragment
IgG1, kappa
Specificity
Does not react with bacteria, helminths, spinach.
Applications Notes
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
HSPD1
NCBI Official Name
Heat shock 60kDa protein 1 (chaperonin)
NCBI Organism
Homo sapiens
Other Product Names
60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60
Tested Applications
WB, Flow, IHC, IF
Physical Properties
Liquid
Curated Selection
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