HSP27 Antibody [G3.1]

Product Specifications

Background

This antibody recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27) . HSP27 was recently found to be identical to the estrogen-induced “p29” and “24K” protein. About 50% of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

NCBI Gene ID

3315

Swiss Prot

P04792

Host

Mouse

Reactivity

Human, Chimpanzee, Sheep, Rat, Mouse, Chicken

Immunogen

Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

G3.1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle, Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG1, kappa

Applications Notes

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Symbol

HSPB1

NCBI Official Name

Heat shock 27kDa protein 1

NCBI Organism

Homo sapiens

Other Product Names

Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28