Bcl-X Antibody [BX006]

Product Specifications

Background

This antibody recognizes a protein of 27kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

NCBI Gene ID

598

Swiss Prot

Q07817

Host

Mouse

Reactivity

Human, Mouse, Rat, Pig

Immunogen

Amino acids QSNRELVVDFLS (3-14) of the human protien were used as the immunogen for this Bcl-X antibody (2) .

Clonality

Monoclonal

Clone

BX006

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG2a

Applications Notes

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. 2. This antibody reacts with both BclXS and BclXL. 3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.