Alpha Fetoprotein Antibody [C3]

Product Specifications

Background

This antibody recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as Alpha fetoprotein (AFP) . The AFP antibody is highly specific and shows no cross-reaction with other oncofetal antigens or serum albumin. Alpha fetoprotein is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. AFP antibody has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

NCBI Gene ID

174

Swiss Prot

P02771

Host

Mouse

Reactivity

Human, Monkey, Dog, Pig

Immunogen

Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.

Clonality

Monoclonal

Clone

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Buffer

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Fragment

IgG2a, kappa

Specificity

Does not react with cow, cat, mouse, rat.

Applications Notes

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.