SPPL2B Antibody

Product Specifications

Background

SPPL2B is a member of the GXGD family of aspartic proteases. The GXGD proteases are transmembrane proteins with two conserved catalytic motifs localized within the membrane-spanning regions. This enzyme localizes to endosomes, lysosomes, and the plasma membrane. It cleaves the transmembrane domain of tumor necrosis factor alpha to release the intracellular domain, which triggers cytokine expression in the innate and adaptive immunity pathways.

NCBI Gene ID

56928

Swiss Prot

Q8TCT7-4

Accession Number

NP_001070706

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPPL2B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Membrane, Cancer

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SPPL2B antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

56 kDa, 65 kDa

Applications Notes

SPPL2B antibody can be used for detection of SPPL2B by ELISA at 1:312500. SPPL2B antibody can be used for detection of SPPL2B by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.