NUCB1 Antibody
Product Specifications
Background
An extracellular calcium binding protein of the mineralized matrix of bone [Calnuc or RGD:620030]. Also useful as a Golgi marker in immunohistochemistry (1:100 dilution) .
NCBI Gene ID
84595
Swiss Prot
Q63083
Accession Number
NP_445915
Host
Rabbit
Reactivity
Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic KLH-conjugated peptide corresponding a region of rat NUCB1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Signal Transduction
Purification
Antibody is purified by Protein A/G affinity chromatography method.
Positive Control
Cat. No. 1201 - HeLa Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NUCB1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
54 kDa
Applications Notes
NUCB1 antibody can be used for detection of NUCB1 by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
NUCB1
NCBI Official Name
Nucleobindin 1
NCBI Organism
Rattus norvegicus
Background Reference 01
Wendel, M., et al., 1995, J. Biol. Chem. 270 (11), 6125-6133.
Other Product Names
NUCB1, Nucb
Tested Applications
ELISA, WB, IHC, IP
Protein ID
16758210
Physical Properties
Liquid
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