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PC4 Antibody

Product Specifications

Background

PC4 (activated RNA polymerase II transcription cofactor 4) is a transcriptional coactivator, possessing the ability to suppress promoter-driven as well as nonspecific transcription via its DNA binding activity. The repressive activity of PC4 on promoter-driven transcription is alleviated by transcription factor TFIIH. TFIIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity.

NCBI Gene ID

10923

Swiss Prot

P53999

Accession Number

NP_006704

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Zebrafish

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PC4.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is supplied as total IgG.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PC4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

14 kDa

Applications Notes

PC4 antibody can be used for detection of PC4 by ELISA at 1:7812500. PC4 antibody can be used for detection of PC4 by western blot at 0.5-1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

SUB1

NCBI Official Name

SUB1 homolog (S. cerevisiae)

NCBI Organism

Homo sapiens

Background Reference 01

Banerjee, S., et al., (2004) Cell.Biol.24 (5), 2052-2062.

Other Product Names

PC4, P15, PC4, p14

Tested Applications

ELISA, WB, IHC

Protein ID

217330646

Physical Properties

Liquid

Frequently Asked Questions

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