E2F5 Antibody

E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store E2F5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

E2F5 antibody can be used for detection of E2F5 by ELISA at 1:1562500. E2F5 antibody can be used for detection of E2F5 by western blot at 0.4 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.